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Table 1 Uncertainties from the experimental calibration curve calculated because the arithmetic indicate from eight calibration curves equivalent to PUFA methyl ester specifications.

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In the above mentioned studies, the extrapolation from the response variable didn't keep in mind the dependence on the instrument response on chain duration, nor the degree as well as situation of unsaturations. Liu et al.14 created a technique to quantify VLC-PUFA expressed as fatty acids molar share, though the reaction of only one quadrupole mass analyzer demonstrates traits because of chain size, variety of unsaturations and their position, and the necessity of undertaking extrapolations.

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